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The various steps for construction of libraries are ___________ i) Fragmentation of DNA ii) Isolation of genomic DNA iii) Amplification iv) Ligation and introduction to the host v) Vector preparation The correct order of construction of libraries is (In the order of starting to ending).

Correct Answer: ii)-i)-v)-iv)-iii)
Firstly, the isolation of genomic DNA is carried out. It is followed by the fragmentation of DNA. Then the vector preparation is done, which is followed by the ligation and introduction to the host. The final step is the amplification of the recombinants.

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps: i) Peeling of membranes carrying away bacterial cells with it ii) Cells are lysed and denaturing of DNA is being carried out iii) Hybridization with labelled DNA iv) Placing the membrane onto plate containing recombinant cells Choose the correct sequence in which the steps are carried out (starting to ending).

Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor

Three cats are roaming in a zoo n such a way that when cat A takes 5 steps, B takes 6 steps and C takes 7 steps.But the 6 steps of A are equal to the 7 steps of B and 8 steps of C. what is the ratio of their speeds?

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it. The Alaska pipeline starts at the frozen edge of the Arctic Ocean. It stretches southward across the largest and northernmost state in the United States, ending at a remote ice-free seaport village nearly 800 miles from where it begins. It is massive in size and extremely complicated to operate. The steel pipe crosses windswept plains and endless miles of delicate tundra that tops the frozen ground. It weaves through crooked canyons, climbs sheer mountains, plunges over rocky crags, makes its way through thick forests, and passes over or under hundreds of rivers and streams. The pipe is 4 feet in diameter, and up to 2 million barrels (or 84 million gallons) of crude oil can be pumped through it daily. Resting on H-shaped steel racks called "bents", long sections of the pipeline follow a zigzag course high above the frozen earth. Other long sections drop out of sight beneath spongy or rocky ground and return to the surface later on. The pattern of the pipeline's up-and-down route is determined by the often harsh demands of the arctic and subarctic climate, the tortuous lay of the land, and the varied compositions of soil, rock, or permafrost (permanently frozen ground). A little more than half of the pipeline is elevated above the ground. The remainder is buried anywhere from 3 to 12 feet, depending largely upon the type of terrain and the properties of the soil. One of the largest in the world, the pipeline cost approximately $8 billion and is by far the biggest and most expensive construction project ever undertaken by private industry. In fact, no single business could raise that much money, so 8 major oil companies formed a consortium in order to share the costs. Each company controlled oil rights to particular shares of land in the oil fields and paid into the pipeline-construction fund according to the size of its holdings. Today, despite enormous problems of climate, supply shortage, equipment breakdowns, labour disagreements, treacherous terrain, a certain amount of mismanagement, and even theft, the Alaska pipeline has been completed and is operating.
How was the fund for pipeline - construction generated?

These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order? (Mentioned from starting to ending the reaction)

Consider the following statements regarding the general symptoms of diseases and choose the correct answer:
1. The charges in the host which serve to recognise the disease are called the signs and symptoms of the disease
2. The sign of a disease is the external appearance of some portion of the pathogen of the host
3. The symptoms are the visible effects which the host plant induces on the parasites
4. Any visible deviation on the host plant from the normal structure and function is called a symptom

Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it. The Alaska pipeline starts at the frozen edge of the Arctic Ocean. It stretches southward across the largest and northernmost state in the United States, ending at a remote ice-free seaport village nearly 800 miles from where it begins. It is massive in size and extremely complicated to operate. The steel pipe crosses windswept plains and endless miles of delicate tundra that tops the frozen ground. It weaves through crooked canyons, climbs sheer mountains, plunges over rocky crags, makes its way through thick forests, and passes over or under hundreds of rivers and streams. The pipe is 4 feet in diameter, and up to 2 million barrels (or 84 million gallons) of crude oil can be pumped through it daily. Resting on H-shaped steel racks called "bents", long sections of the pipeline follow a zigzag course high above the frozen earth. Other long sections drop out of sight beneath spongy or rocky ground and return to the surface later on. The pattern of the pipeline's up-and-down route is determined by the often harsh demands of the arctic and subarctic climate, the tortuous lay of the land, and the varied compositions of soil, rock, or permafrost (permanently frozen ground). A little more than half of the pipeline is elevated above the ground. The remainder is buried anywhere from 3 to 12 feet, depending largely upon the type of terrain and the properties of the soil. One of the largest in the world, the pipeline cost approximately $8 billion and is by far the biggest and most expensive construction project ever undertaken by private industry. In fact, no single business could raise that much money, so 8 major oil companies formed a consortium in order to share the costs. Each company controlled oil rights to particular shares of land in the oil fields and paid into the pipeline-construction fund according to the size of its holdings. Today, despite enormous problems of climate, supply shortage, equipment breakdowns, labour disagreements, treacherous terrain, a certain amount of mismanagement, and even theft, the Alaska pipeline has been completed and is operating.
The Alaskan pipeline ends