The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps: i) Peeling of membranes carrying away bacterial cells with it ii) Cells are lysed and denaturing of DNA is being carried out iii) Hybridization with labelled DNA iv) Placing the membrane onto plate containing recombinant cells Choose the correct sequence in which the steps are carried out (starting to ending).

The various steps for construction of libraries are ___________ i) Fragmentation of DNA ii) Isolation of genomic DNA iii) Amplification iv) Ligation and introduction to the host v) Vector preparation The correct order of construction of libraries is (In the order of starting to ending).

A

i)-ii)-iii)-iv)-v)

B

ii)-i)-v)-iv)-iii)

C

ii)-v)-i)-iv)-iii)

D

v)-ii)-i)-iii)-iv)

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

A

3, 1, 2, 4, 6, 5, 7, 8

B

1, 4, 3, 2, 5, 7, 6, 8

C

1, 2, 6, 7, 5, 8, 3, 4

D

8, 6, 7, 5, 1 , 4, 2, 3

Consider the following statements regarding the historical aspects of DNA and choose the correct answer:
1. Meischer isolated nucleic acids, which he called nuclein
2. Altmann used the term nucleic acid to replace nuclein
3. Fischer identified pyrimidines and purines
4. Griffiths discovered bacterial transformation by DNA

A

Only 1, 2 and 4 are true

B

Only 2, 3 and 4 are true

C

1, 2, 3 and 4 are true

D

Only 1 and 4 are true

Solution-1 containing 10% nitric acid and 20% acetic acid at the rate F combines with Solution-2 containing 20% nitric acid and 10% acetic acid at the rate D, the product formed contains 30% nitric acid and 20% acetic acid at the rate P, how many independent equations containing F, D, and P are possible?

A

1

B

2

C

3

Solution-1 containing 10% hydrochloric acid and 20% acetic acid at the rate F combines with Solution-2 containing 20% hydrochloric acid and 30% acetic acid at the rate D, the product formed contains 20% hydrochloric acid and 30% acetic acid at the rate P, how many independent equations containing F, D, and P are possible?

A

1

B

2

C

3

In a batch process, the reaction takes place in the presence of an acid medium. The acid is drained from the reaction vessel at the rate of 15ml/s as a result of the density difference of the acid from the reacting component. To avoid wastage of acid, it is recycled to an acid tank which has 1000 L capacity. The acid drained from the reaction vessel, picks up 50 g/L solids from the reactor. Acid is fed once again to the process from acid tank. When the process is started, the acid is almost pure in the tank as a result of filtration. As the reaction proceeds, acid in the tank gets more and more contaminated with the solids. The concentration of the solids should not exceed 100 g/L from the process point of view. The batch time is 16h. Estimate whether the concentration of the solids will exceed 100g/L during the batch reaction.

A

37.04 h

B

30.05 h

C

36.04 h

D

32.05 h

If the probability of incorporation of a gene A from a bacterial cell that has lysed in the medium is a. And the probability of incorporation of another gene B from the same bacteria that has lysed is b. What is the probability of one recipient receiving both?

A

a+b

B

a-b

C

a X b

D

a/b

These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order? (Mentioned from starting to ending the reaction)

A

i)-ii)-iii)-iv)

B

ii)-i)-iii)-iv)

C

iv)-iii)-ii)-i)

D

iv)-ii)-i)-iii)

Consider the following statements regarding genetic code and choose the correct answer:
1. The genetic code is degenerate
2. In all amino acids the first two bases are the same in all the codons that represent a particular amino acid
3. The number of codons an amino acid can claim is proportional to the frequency of the amino acid in proteins
4. Amino acids with codons ending in U or A also have codons ending in C or G respectively

A

Only 1, 2 and 3 are true

B

Only 2, 3 and 4 are true

C

Only 1, 3 and 4 are true

D

Only 1 and 4 are true

Which of the following phrases (i),(ii), and (iii) given below each sentenceshould replace the phrase printed in boldletters to make the sentence grammaticallycorrect? Choose the best option among thefive given alternatives that reflect the correctuse of phrase in the context of thegrammatically correct sentence. If thesentence is correct as it is, mark (E) i.e., "Nocorrection required" as the answer. Despite the abuse and insults that werehurled at him, he really sticks on his gunsand showed the protesters how to becalm.
i. he really stuck onto his guns and showedthe protesters
ii. he really stuck to his guns and showed theprotesters
iii. he really is sticking his guns onto theprotesters

A

A. Only i

B

B. Only ii

C

C. Only iii

D

D. ii and iii