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Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

Correct Answer: AMP is added to the 5’ phosphate of one of the DNA molecule
AMP is added to the 5’ phosphate of one of the DNA molecule. It leads to the liberation of pyrophosphate from ATP and nicotanamide mononucleotide from NAD. It is further displaced by nucleophilic attack by 3’ hydroxyl of the other DNA molecule.

Consider the 5 × 5 matrix \[{\text{A}} = \left[ {\begin{array}{*{20}{c}} 1&2&3&4&5 \\ 5&1&2&3&4 \\ 4&5&1&2&3 \\ 3&4&5&1&2 \\ 2&3&4&5&1 \end{array}} \right

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

Let A be an m × n matrix and Ban n × m matrix. It is given that determinant ($$I$$m + AB) = determinant ($$I$$n + BA), where $$I$$k is the k × k identity matrix. Using the above property, the determinant of the matrix given below is
\[\left[ {\begin{array}{*{20}{c}} 2&1&1&1 \\ 1&2&1&1 \\ 1&1&2&1 \\ 1&1&1&2 \end{array}} \right

Eigen values of the matrix \[\left[ {\begin{array}{*{20}{c}} 0&1&0&0 \\ 1&0&0&0 \\ 0&0&0&{ - 2i} \\ 0&0&{2i}&0 \end{array}} \right

If the coli form bacteria is present in a sample of water, then the coli-form test to be conducted is
(i) Presumptive coli-form test
(ii) Confirmed coli-form test
(iii) Completed coli-form test

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

Three cats are roaming in a zoo n such a way that when cat A takes 5 steps, B takes 6 steps and C takes 7 steps.But the 6 steps of A are equal to the 7 steps of B and 8 steps of C. what is the ratio of their speeds?

Adenosine deaminase deaminates adenosine to ____________

The minimum and the maximum eigen values of the matrix \[\left[ {\begin{array}{*{20}{c}} 1&1&3 \\ 1&5&1 \\ 3&1&1 \end{array}} \right

The smallest and largest Eigen values of the following matrix are \[\left[ {\begin{array}{*{20}{c}} 3&{ - 2}&2 \\ 4&{ - 4}&6 \\ 2&{ - 3}&5 \end{array}} \right