Consider the following statements. I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase. II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

AMP is added to the 5’ phosphate of one of the DNA molecule

It leads to the liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP

The AMP is further displaced by an electrophilic attack

The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule

If the coli form bacteria is present in a sample of water, then the coli-form test to be conducted is
(i) Presumptive coli-form test
(ii) Confirmed coli-form test
(iii) Completed coli-form test

Only (i)

Both (i) and (ii)

Both (i) and (iii)

All (i), (ii) and (iii)

In the case of blunt-end ligation, blunt ends can be generated by ___________

simply the action of restriction endonuclease which gives straight ends

the polishing of staggered ends

both the action of restriction endonuclease which gives straight ends and polishing of staggered ends

by the action of restriction endonuclease which gives staggered ends

Consider the following statements and choose the correct answer:
1. In order to increase the photosynthetic efficiency leaf area index should be increased
2. To increase leaf area index plants should be with horizontal leaves
3. If the time of grain-growth is increased, the grain-yield will decrease
4. Photosynthetic efficiency can be increased by monitoring moisture stress, transpiration loss and light intensity

1 and 3 are true

2 and 4 are true

1 and 4 are true

3 and 4 are true

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

3, 1, 2, 4, 6, 5, 7, 8

1, 4, 3, 2, 5, 7, 6, 8

1, 2, 6, 7, 5, 8, 3, 4

8, 6, 7, 5, 1 , 4, 2, 3

Output of a bulldozer is
(i) Increased if drawbar HP of the tractor is increased for a given hauling distance
(ii) Decreased if drawbar HP of the tractor is increased for a given hauling distance
(iii) Increased if the hauling distance is increased for a given drawbar HP of the tractor
(iv) Decreased if the hauling distance is increased for a given drawbar HP of the tractor

(i) and (iii) are correct

(i) and (iv) are correct

(ii) and (iii) are correct

(ii) and (iv) are correct

L is older than M and taller than N. O is younger than P, older than N and shorter than Q. R is older than S, younger than N, shorter than S and taller than Q. S is older than L, M, O, P, Q, R and S is necessarily false?

O is the shortest of the group.

N is the second tallest of the group.

L is the tallest of the group.

Q is the third oldest of the group.

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

1, 2, 3 and 4 are true

Only 2 and 4 are true

Oniy 2, 3 and 4 are true

Only 1 and 2 are true

Find the appropriate relation for quantity1 and quantity2 in the following question: Quantity 1: In an examination, Ankita scored 35 marks less than Puneeta. Puneeta scored 65 more marks than Meenakshi. Rakhi scored 115 marks, which is 20 marks more than Meenakshi's. Simpy scored 108 marks less than the maximum marks of the test. What approximate percentage of marks did Simpy score in the examination, if she got 67 marks more than Ankita? Quantity 2: The length of a rectangle is increased by 60%. By what percent would the width have to be decreased to maintain the same area?

Quantity1 < Quantity2

Quantity1 \u2264 Quantity2

Quantity1 \u2265 Quantity2

Quantity1 > Quantity2

DNA ligase of E. coli requires which of the following cofactors?

Flavin-adenine dinucleotide as an electron acceptor

Nicotinamide-adenine dinucleotide phosphate as a phosphate donor

Nicotinamide-adenine dinucleotide (NAD+) to form an active adenyl enzyme

(NAD+) as an electron acceptor