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Consider the following statements regarding the DNA-mediated gene transfer and choose the correct answer:<br>1. DNA fragments carrying the gene to be transferred are mixed with a carrier DNA<br>2. They are then precipitated out with a calcium phosphate buffer<br>3. The precipitate is used to transform target cells<br>4. The Ca-precipitated DNA enters the cell by exocytosis

Correct Answer: Only 1, 2 and 3 are true

Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

Laplace transform of the function f(t) is given by $${\text{F}}\left( {\text{s}} \right) = {\text{L}}\left\{ {{\text{f}}\left( {\text{t}} \right)} \right\} = \int_0^\infty {{\text{f}}\left( {\text{t}} \right){{\text{e}}^{ - {\text{st}}}}{\text{dt}}{\text{.}}} $$       Laplace transform of the function shown below is given by
Transform Theory mcq question image

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

Consider the following statements regarding cell cycle and choose the correct answer:
1. During G1 phase the cell grows in size and there is an active synthesis of RNA and proteins
2. The third stage of the cell cycle is termed G2 phase
3. In the mitotic phase, the DNA molecule of each chromosome replicates by the synthesis of a new DNA molecule on the template of the existing DNA

A GSM cellular telephone standard requires a minimum of 9 dB rejection of interfering signal levels of -23 dBm at 3 MHz from the carrier, -33 dBm at 1.6 MHz from the carrier, and -43 dBm at 0.6 MHz from the carrier, for a carrier level of -99 dBm. Determine the required local oscillator phase noise at 3 MHz carrier frequency offset.

Consider the following statements.
1. Golden rice is vitamin A rich.
2. Replacement of faulty gene by normal gene is called gene therapy.
3. Transgenic organism carrying introduced foreign gene also called genetically modified organism.
Which of the statements given above are true?

Consider the following statements and choose the correct answer:
1. Autophagy is the digestion of endogenous material
2. Heterophagy is the intake of exogenous material into the cell and the subsequent breakdown of this material by enzymes
3. Both pinocytosis and phagocytosis require energy
4. The bulk intake of exogenous material is called exocytosis

The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.

Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it.
Modern civilization is completely dependent on energy, which has therefore to be abundant and also economical. About 85% of the world's energy is supplied by oil, coal and natural gas while nuclear, hydro, wind and solar power and biomass supply the rest. Coal, nuclear and hydro are used primarily to generate electricity while natural gas is widely used for heating. Biomass is used both for heating and cooking. The wind and solar power is the future's hope as they are sustainable energy sources. Oil powers almost all machines that move and that makes oil uniquely versatile. Oil powered airplanes carry 500 people across the widest oceans at nearly the speed of sound. Oil powered machines produce and transport food. Oil powered machines are ubiquitous. Clearly, we live in the age of oil but it is drawing to a close. According to data available if oil production remains constant until it's gone, there is enough to last 42 years. Oil wells will produce less as they become depleted, which will make it impossible to keep production constant. Similarly natural gas and coal will last another 61 years and 133 years respectively. Naturally, as they become scarce, they become expensive, leading to a worldwide energy crisis. If we are to survive on this planet, we have to make a transition to sustainable energy sources. The transition may be willy-nilly or planned - the choice is ours. The dawning era of limited and expensive energy will be very difficult for everyone on earth but will be even more difficult if it is not anticipated. It is of utmost importance that the public and policymakers understand the global energy crisis and act in tandem to ensure that the species 'homo sapiens' does not become extinct. Biomass is an energy source used in