Thermal death of microorganisms in the liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 10<sup>8</sup> cells mL<sup>-1</sup> and the final acceptable contamination level is 10<sup>-3</sup> cells, for how long should 1 m<sup>3</sup> medium be treated at temperature of 120°C (thermal deactivation rate constant = 0.23 min<sup>-1</sup>) to achieve acceptable load?

Correct Answer: 110 min