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What are the experimental steps needed for screening an expression library for clone encoding a protein X that has been isolated and purified?<br>P. mRNA isolation<br>Q. Antibody preparation<br>R. Cloning into an appropriate vector<br>S. Western blotting on transferred plaques

Correct Answer: Q, R

Jane, Karim and Leo checked out books from the library. Jane has an even number of library books . Karim has 3 more library books than Jane . Leo has 2 more library books than Karim. Which statement is true about the number of library books Karim and Leo have ?

The gene of interest is inserted into transfer vector and then further into viral genome leading to the formation of modified virus. How can plaques from modified virus differentiated from plaques from wild-type virus?

Three cats are roaming in a zoo n such a way that when cat A takes 5 steps, B takes 6 steps and C takes 7 steps.But the 6 steps of A are equal to the 7 steps of B and 8 steps of C. what is the ratio of their speeds?

A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

Mr. Bakhtiar works in the procurement department of a library. Under a new purchase policy, he increased the total number of books in the library by 20%. But soon afterwards, as part of the library cleaning process, 10% of the total library book stock is disposed of. What is the net change over the initial number of books in the library ?

Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:

The protein bands transferred by the western blotting are previously ______________

Directions : In each of the following questions a statement is given, followed by two conclusions. Give answer :

Statements : About 50 per cent of the animal by-products - hair, skin, horns etc. is edible protein. American chemists have developed a method of isolating 45 per cent of this protein. They used an enzyme developed in Japan to break down soya protein.

Conclusions :
I. Americans have not been able to develop enzymes.
II. Animal by-products protein has the same composition as soya protein.

In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.

In the following passage some of the words have been left out. Read the passage carefully and choose the correct answer for the given blank out ofthe four alternatives.
We have rich cultural _____ . Its roots go into _____. Ours has never been a closed ______. It has _______ just like a tree, ______ to external ______ but holding itsroots _______. But one wonders today whether it will be able to hold its _____. The ______ of our cultural heritage has come under the _____ of western culture.
the _____ of western culture.