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The protein bands transferred by the western blotting are previously ______________

Correct Answer: Electrophoresed
Western blotting involves a transfer of electrophoresed protein bands from a polyacrylamide gel on to a nitrocellulose or nylon membrane.

Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:

What are the experimental steps needed for screening an expression library for clone encoding a protein X that has been isolated and purified?
P. mRNA isolation
Q. Antibody preparation
R. Cloning into an appropriate vector
S. Western blotting on transferred plaques

Consider the following statements regarding bands to be provided for strengthening masonry work in masonry buildings constructed in zone III, IV and V.
(i) Lintel band is provided at lintel level on partition walls,
(ii) Gable band is provided at top of gable masonry below the purlins,
(iii) The bands shall be to full width of the wall and not less than 7.5 cm in depth,
(iv) The bands shall be made of reinforced concrete only.
of these statements, the correct statements are

Directions : In each of the following questions a statement is given, followed by two conclusions. Give answer :

Statements : About 50 per cent of the animal by-products - hair, skin, horns etc. is edible protein. American chemists have developed a method of isolating 45 per cent of this protein. They used an enzyme developed in Japan to break down soya protein.

Conclusions :
I. Americans have not been able to develop enzymes.
II. Animal by-products protein has the same composition as soya protein.

Northern Blotting is not a variant of Southern blotting.

Which of the following is found in Northern blotting but not in southern blotting?

The question below consists of a set of labelled sentences. Out of the four options given, select the most logical order of thesentences to form a coherent paragraph.
P- Epistemological idealists (such as Kant) might insist that the only things which can be directly known for certain are ideas.
Q- The approach to idealism by Western philosophers has been different to that of Eastern thinkers.
R- In much of Western thought (though not in such major Western thinkers as Plato and Hegel) the ideal relates to direct knowledge of
subjective mental ideas, or images.
S- It is then usually juxtaposed with realism in which the real is said to have absolute existence prior to and independent of our knowledge.

Protein A will fold into its native state only when protein B is also present in the solution. However, protein B can fold itself into native confirmation without the presence of protein A. Which of the following is true?

Protein’s solubility depends on the relative balance between protein-solvent and protein-protein interactions.

Inhomogeneous deformation of crystal results in regions of different orientation called deformation bands. Deformation bands have been observed in both fcc and bcc metals, but not in hcp metals.