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Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm<sup>-1</sup>. At a concentration of 50 mg L<sup>-1</sup> of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm<sup>-1</sup>. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L<sup>-1</sup> protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
A
21.4 mg cm<sup>-3</sup>
B
214 mg cm<sup>-3</sup>
C
2.14 mg cm<sup>-3</sup>
D
None of the above
Correct Answer:
None of the above
Adsorption of methane follows the Langmuir adsorption isotherm at 90K. If p = 1.896cm3g-1bar-1 and b = 0.146bar-1. Calculate the value of θ.
A
0.116 bar
B
0.514 bar
C
0.214 bar
D
0.216 bar
A continuous process is set up for treatment of wastewater. Each day, 103 kg cellulose and 105 kg bacteria enter in the feed stream, while 102 kg cellulose and 1.5 x 102 kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 8 x 102 kg d-1. The rate of bacterial growth is 4x 102 kg d-l; the rate of cell death by lysis is 6 x 102 kg d-1. Write balances for cellulose and bacteria in the system.
A
1× 103 kg, 9 × 103 kg
B
1× 102 kg, 9.965 × 104 kg
C
1× 103 kg, 9.964 × 104 kg
D
1× 102 kg, 9 × 103 kg
Ahar culture, Kayatha culture, Malwa culture, Savalda culture, Jorwa culture, Prabas culture, Rangpur culture are all parts of:
A
Lower Paleolithic Cultures
B
Middle Paleolithic Cultures
C
Upper Paleolithic Cultures
D
Chalcolithic Cultures
Ahar culture, Kayatha culture, Malwa culture, Savalda culture, Jorwe culture, Prabhas culture, Rangpur culture are all :
A
Mesolithic cultures
B
Palaeolithic cultures
C
The Chalcolithic cultures of Ancient India
D
None of these
Which type of adsorption follows Langmuir isotherm?
A
Chromatography
B
Ion exchange chromatography
C
Affinity chromatography
D
Ion exchange and affinity chromatography
Consider a given volume of liquid at a temperature T below Tm with a free energy G’. If some of the atoms of the liquid cluster together to form a small sphere of solid, the free energy of the system will change to G, given by________ (Assume V1 volume of the solid sphere, V2 volume of liquid, A is the solid/liquid interfacial area, γ solid/liquid interface free energy, G1 and G2 are the free energies per unit volume of solid and liquid respectively).
A
V1G1 + V2G2 + Aγ
B
V1G2 + V2G1 + Aγ
C
V1G1 + V2G2 – Aγ
D
V1G2 + V2G1 – Aγ
Bacillus subtilis under ideal conditions occupies 5% of the total volume of a fermenter. Under such conditions; the culture medium (after bacilli are filtered off) has a concentration of 1mg/ml of the secreted protein trehalae
(i) Calculate the volume of culture filtrate required to purify 1 kg of trehalase
(ii) What are the total number of B. subtilis cells which produce this amount of enzyme? Assume that each cell is a cylinder which has dimensions of 1 m diameter and length 2μm.
A
(i) 10<sup>6</sup> ml, (ii) 1.57 × 10<sup>8</sup>
B
(i) 10<sup>8</sup> ml, (ii) 3.16 × 10<sup>18</sup>
C
(i) 10<sup>6</sup> ml, (ii) 1.57 × 10<sup>8</sup>
D
(i) 10<sup>6</sup> ml, (ii) 3.18 × 10<sup>16</sup>
The change in enthalpy per unit weight of adsorbed gas when adsorbed on gas free or "outgassed" adsorbent to from a definite concentration of adsorbate is called its
A
Integral heat of adsorption
B
Heat of wetting
C
Differential heat of adsorption
D
Heat of normal condensation
Which of the following is not a part of enzyme preparation produced from Aspergillus Niger?
A
Polygalacturonase
B
Pectinesterase
C
Cellulase
D
Hemicellulase
For an adsorbant-adsorbate system obeying the Langmuir adsorption isotherm, b = 0.48 bar-1 and p = 0.16 bar-1. At what pressure will 50% of the surface be covered?
A
0.05 bar
B
0.07 bar
C
0.08 bar
D
0.04 bar