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Which factor phosphorylates the serine residues in RNA pol II CTD?

Correct Answer: TFIIH
TFIIH phosphorylates the serine 2 of the heptad repeat that drives the RNA pol into initiation. The other factors mentioned are only part of pre-initiation complex and not of transcribing RNA pol.

In a Drosophila polytene chromosome just before molting you add green labelled antibody against unphosphhorylated RNA pol II CTD and red labelled antibody against phosphorylated RNA pol II CTD. These give fluorescence on adding the respective substrates. What will be the colour in gene 74 EF and 75 B?

If you consider a transcribing RNA pol II with a transcript as small as 30 bases, which serine residue in its CTD will be phosphorylated?

While in vitro transcription your RNA pol is stalled at a position due to mistreatment. Which factor could you act to help the RNA pol overcome the stalled stage?

In what sequence are the serine residues in RNA pol II phosphorylated?

You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.

In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?

The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

An in vitro translation system can synthesize peptides in all three reading frames of the RNA template. When 5' - UCUCUCUC-(UC)n-UCUCUCUC - 3' was used as the template in this in vitro translation system, the synthesized peptides contained 50% each of serine and leucine. When 5' - CCUCCUCCU-(CCU)n-CCUCCU - 3' was used as the template, the synthesized peptides contained 33.3% each of serine, leucine, and proline. Deduce the codon for proline.

Consider the following statements.
1. The smallest RNA is t RNA.
2. The most abundant RNA of cell is r RNA.
3. DNA can be formed over RNA through reverse transcriptase.
Which of the statements given above are false?