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If you consider a transcribing RNA pol II with a transcript as small as 30 bases, which serine residue in its CTD will be phosphorylated?

Correct Answer: 5
when RNA transcript is only 30 bases long it is still within the initiation range. Then the 2nd serine residue is phosphorylared only.

In a Drosophila polytene chromosome just before molting you add green labelled antibody against unphosphhorylated RNA pol II CTD and red labelled antibody against phosphorylated RNA pol II CTD. These give fluorescence on adding the respective substrates. What will be the colour in gene 74 EF and 75 B?

In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?

You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with

Which factor phosphorylates the serine residues in RNA pol II CTD?

In what sequence are the serine residues in RNA pol II phosphorylated?

While in vitro transcription your RNA pol is stalled at a position due to mistreatment. Which factor could you act to help the RNA pol overcome the stalled stage?

Protein P becomes functional upon phosphorylation of a serine residue, replacing this serine with . . . . . . . . will result in a phosphomimic mutant of P.

Consider the following statements.
1. The smallest RNA is t RNA.
2. The most abundant RNA of cell is r RNA.
3. DNA can be formed over RNA through reverse transcriptase.
Which of the statements given above are false?

An in vitro translation system can synthesize peptides in all three reading frames of the RNA template. When 5' - UCUCUCUC-(UC)n-UCUCUCUC - 3' was used as the template in this in vitro translation system, the synthesized peptides contained 50% each of serine and leucine. When 5' - CCUCCUCCU-(CCU)n-CCUCCU - 3' was used as the template, the synthesized peptides contained 33.3% each of serine, leucine, and proline. Deduce the codon for proline.