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In an experiment you use DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.

Correct Answer: DNA produced will be shorter
Klenow fragment provides the 5’ -> 3’ exonuclease activity used to remove the primer. In its absence the primer remains and hence DNA produced is shorter (i.e. original length-length of primer).

You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?

Which among the following statements regarding filing Rejoinder/Replication in a suit, as per the Code of Civil Procedure 1908, are correct?
1. Rejoinder/ Replication is filed as a matter of Right
2. Provision of filing Rejoinder/ Replication is contained under Order VIII of the Code of Civil Procedure
3. Rejoinder/Replication may be filed only after due permission of the Court.
4. Rejoinder/Replication is a part of pleading.
Select the correct answer:

Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease

You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it.
Ants have been living on the earth for more than 100 million years and can be found almost anywhere on the planet. It is estimated that there are about 20000 different species of ants. For this reason ants have been called Earth's most successful species. If you watch ants for any length of time you will see that they really do communicate with each other and very effectively too. Ants communicate by touching each other with their antennae. Ants also use chemicals called pheromones to leave scent trails for other ants to follow. Ants build many different types of homes. Many ants build simple little mounds out of dirt or sand. Other ants use small sticks mixed with dirt and sand to make a stronger mound that offers protection from rain. Western Harvester ants make a small mound on top, but then tunnels up to 15 feet straight down to hibernate during winter. Ant mounds consist of many chambers connected by tunnels. Different chambers are used for nurseries, food storage, and resting places for the worker ants. Some ants live in wood like termites. Army ants don't make a home at all but travel in a large groups searching for food. Ants are social insects which means they live in large colonies or groups. Some colonies consist of millions of ants. There are three types of ants in each species, the queen, the sterile female workers and males. The male ants only serve one purpose, to mate with future queen ants and do not live very long. The queen grows to adulthood, mates, and then spends rest of her life laying eggs. A colony may have only one queen, or there may be many queens depending on the species. Ants go through four stages of development: egg, larva, pupa and adult.
Why are ants called the 'Earth's' most successful species?

Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it.
Ants have been living on the earth for more than 100 million years and can be found almost anywhere on the planet. It is estimated that there are about 20000 different species of ants. For this reason ants have been called Earth's most successful species. If you watch ants for any length of time you will see that they really do communicate with each other and very effectively too. Ants communicate by touching each other with their antennae. Ants also use chemicals called pheromones to leave scent trails for other ants to follow. Ants build many different types of homes. Many ants build simple little mounds out of dirt or sand. Other ants use small sticks mixed with dirt and sand to make a stronger mound that offers protection from rain. Western Harvester ants make a small mound on top, but then tunnels up to 15 feet straight down to hibernate during winter. Ant mounds consist of many chambers connected by tunnels. Different chambers are used for nurseries, food storage, and resting places for the worker ants. Some ants live in wood like termites. Army ants don't make a home at all but travel in a large groups searching for food. Ants are social insects which means they live in large colonies or groups. Some colonies consist of millions of ants. There are three types of ants in each species, the queen, the sterile female workers and males. The male ants only serve one purpose, to mate with future queen ants and do not live very long. The queen grows to adulthood, mates, and then spends rest of her life laying eggs. A colony may have only one queen, or there may be many queens depending on the species. Ants go through four stages of development: egg, larva, pupa and adult. Antennae are used by ants to

Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it.
Ants have been living on the earth for more than 100 million years and can be found almost anywhere on the planet. It is estimated that there are about 20000 different species of ants. For this reason ants have been called Earth's most successful species. If you watch ants for any length of time you will see that they really do communicate with each other and very effectively too. Ants communicate by touching each other with their antennae. Ants also use chemicals called pheromones to leave scent trails for other ants to follow. Ants build many different types of homes. Many ants build simple little mounds out of dirt or sand. Other ants use small sticks mixed with dirt and sand to make a stronger mound that offers protection from rain. Western Harvester ants make a small mound on top, but then tunnels up to 15 feet straight down to hibernate during winter. Ant mounds consist of many chambers connected by tunnels. Different chambers are used for nurseries, food storage, and resting places for the worker ants. Some ants live in wood like termites. Army ants don't make a home at all but travel in a large groups searching for food. Ants are social insects which means they live in large colonies or groups. Some colonies consist of millions of ants. There are three types of ants in each species, the queen, the sterile female workers and males. The male ants only serve one purpose, to mate with future queen ants and do not live very long. The queen grows to adulthood, mates, and then spends rest of her life laying eggs. A colony may have only one queen, or there may be many queens depending on the species. Ants go through four stages of development: egg, larva, pupa and adult. What does the Western Harvester do in winter ?

Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it.
Ants have been living on the earth for more than 100 million years and can be found almost anywhere on the planet. It is estimated that there are about 20000 different species of ants. For this reason ants have been called Earth's most successful species. If you watch ants for any length of time you will see that they really do communicate with each other and very effectively too. Ants communicate by touching each other with their antennae. Ants also use chemicals called pheromones to leave scent trails for other ants to follow. Ants build many different types of homes. Many ants build simple little mounds out of dirt or sand. Other ants use small sticks mixed with dirt and sand to make a stronger mound that offers protection from rain. Western Harvester ants make a small mound on top, but then tunnels up to 15 feet straight down to hibernate during winter. Ant mounds consist of many chambers connected by tunnels. Different chambers are used for nurseries, food storage, and resting places for the worker ants. Some ants live in wood like termites. Army ants don't make a home at all but travel in a large groups searching for food. Ants are social insects which means they live in large colonies or groups. Some colonies consist of millions of ants. There are three types of ants in each species, the queen, the sterile female workers and males. The male ants only serve one purpose, to mate with future queen ants and do not live very long. The queen grows to adulthood, mates, and then spends rest of her life laying eggs. A colony may have only one queen, or there may be many queens depending on the species. Ants go through four stages of development: egg, larva, pupa and adult. What kind of homes do the army of ants build ?