How did Alfred Hershey and Martha Chase arrive at the conclusion that DNA is the genetic material ?

Monjur Alahi

How did Alfred Hershey and Martha Chase arrive at the conclusion that DNA is the genetic material ?


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Salim Ahmed Kawsar

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Hershey - Chase experiment.

Hershey and Chase grew some viruses in a medium that contained 32P radioactive phosphorus, these were allowed to infect E. coli, medium was agitated in a blender. Viral coats and the bacterial cells with viral particles were separated by spinning them in a centrifuge. In this case no radioactivity in the supernatant as the protein coats do not incorporate 32P, but the viral DNA had 32P and passed it to the bacterial cell, so radioactivity was detected in the cells, proves that DNA is the hereditary material, They repeated the procedure with radioactive sulphur 35S, in this case no radioactive  was detected in the bacterial cell as S is not incorporated in DNA, while radioactivity was detected in the supernatant with protein coats of viruses.

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