Write the steps of BLAST involved in comparison of DNA sequences.

Monjur Alahi

Write the steps of BLAST involved in comparison of DNA sequences.


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Salim Ahmed Kawsar

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  • The given sequence is compared with sequences in the database using substitution matrices that specify scores to either 'reward' a match or 'penalize' a mismatch. 
  • Top scoring matches are ranked according to set criteria that serve to distinguish between a similarity due to ancestral relationship or due to random chance. 
  • True matches are further examined thoroughly with other details accessible through Entrez and other tools available at NCBI.

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