Why is r-HUEPO preferred over blood transfusion in such cases where a person has excessive blood loss due to accidents? 

OR 

Differentiate between primary and secondary animal cell cultures.


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No donor is required for transfusion, no transfusion facilities, no risk of transfusion related infection (any two) 

OR 

The maintenance of growth of cells under laboratory conditions in suitable culture medium is known as primary cell culture. 

The primary cell culture is sub-cultured in fresh growth media to develop secondary cultures.

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