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cDNA made from the mRNA of an organism was used to make a cDNA library in a vector that allows the expression as a fusion with a reporter tag. What percentage of the cDNA clones is likely to give rise to correct gene products?

Correct Answer: 50%

A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).

For gene trap vector system, reporter gene and _____ are present. Reporter gene is _____

You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

Consider the following statements.
1. Golden rice is vitamin A rich.
2. Replacement of faulty gene by normal gene is called gene therapy.
3. Transgenic organism carrying introduced foreign gene also called genetically modified organism.
Which of the statements given above are true?

Jane, Karim and Leo checked out books from the library. Jane has an even number of library books . Karim has 3 more library books than Jane . Leo has 2 more library books than Karim. Which statement is true about the number of library books Karim and Leo have ?

Mr. Bakhtiar works in the procurement department of a library. Under a new purchase policy, he increased the total number of books in the library by 20%. But soon afterwards, as part of the library cleaning process, 10% of the total library book stock is disposed of. What is the net change over the initial number of books in the library ?

Expression in poor amount and in inactive form of cDNA of a eukaryotic protein in Escherichia coli using its expression vector is due to
P. The absence of capping mechanism of mRNA.
Q. Codon bias.
R. Absence of polyadenylation.
S. Absence of proper glycosylation.

In order to isolate cDNA for a specified protein from a mice cDNA library prepared in λgt11 vector which of the following methods you will choose for screening?

Which of the following statements about gene therapy are correct?
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.