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cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).

Correct Answer: True
There has been a great deal of progress in developing computational methods for analyzing genomic sequences and finding these protein-encoding regions. But these methods are not completely reliable and, furthermore, such genomic sequences are often not available.

A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction?

A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

One copy machine can make 20 copies a minute, and a second copy machine makes 15 copies a minute. If the two copies work together, how long would it take them to make 2100 copies?

At a photocopy center , the first 10 copies cost x taka each. Each of the next 50 copies costs 2 taka less per copy . From the 61 st copy on, the cost 1 Taka per copy, In terms of x how much does it cost in. Taka to have 200 copies made/

Statements : Some clips are copies. Some copies are magazines. No magazines is a dictionary

Conclusions :
I. No copies are dictionary.
II. Some copies are dictionary.
III. Some copies are not dictionary.
IV. No clips are magazines.

Under Section 63 of the Indian Evidence Act, secondary evidence includes:
1. Certified copies
2. Copies made from the original by mechanical processes, and copies compared with such copies
3. Copies made from or compared with the original
4. Counterparts of documents, as against the parties who did not execute them
5. Oral accounts of the contents of a document by a person who has seen it

One copy machine can make 20 copies a minute , and a second copy machine makes 15 copies a minute. If the two copiers work together, how long would it take them to make 2100 copies?

To constitute a matter of res judicata which of the following conditions must concur?
1. The matter directly and substantially in issue in the subsequent suit or issue must be the same matter which was directly and substantially in issue either actually (section 11, explanation III) or constructively (section 11, explanation IV) in the former suit
2. The former suit must have been a suit between the same parties under whom they or any of them claim. Explanation VI of Section 11 must be read with this condition
3. The parties as aforesaid must have litigated under the same title in the former suit
4. The court which decided the former suit must have been a court competent to try the subsequent suit of the suit in which such issue has been subsequently raised. Explanation II of section 11 is to be read with condition
5. The matter directly and substantially in issue in the subsequent suit must have been heard and finally decided by the court in the first suit. Explanation V of section 11 is to be read with this condition

The cost of setting up a magazine is Rs. 2800. The cost of paper and ink etc is Rs. 80 per 100 copies and printing cost is Rs. 160 per 100 copies. In last month 2000 copies were printed but only 1500 copies could be sold at Rs. 5 each. Total 25% profit on the sale price was realized. There is one more resource of income from magazine which is advertising. What sum of money obtained from the advertising in magazine?

cDNA made from the mRNA of an organism was used to make a cDNA library in a vector that allows the expression as a fusion with a reporter tag. What percentage of the cDNA clones is likely to give rise to correct gene products?