Get Advice on Live Video Call
Earn $ Cash $ with
consultations on Bissoy App

The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

Correct Answer: All of the mentioned
Helper retrovirus is used for introduction of transfected DNA into mammalian cells. But it is necessary that helper retrovirus itself should not be packaged. It can be achieved by deletion of the packaging signal in the helper genome, deletion of multiple parts of the helper genome and division of the helper genome into several pieces.

Which of the following statement(s) is/are CORRECT for antigen activated effector T cells?
P. CD4+ cells make contact with macrophages and stimulate their microbicidal activity.
Q. CD4+ cells make contact with B cells and stimulate them to differentiate into plasma cells.
R. CD8+ cells make contact with B cells and stimulate them to differentiate into plasma cells.
S. CD8+ cells make contact with virus infected cells and kill them.

If the cells containing plasmids are infected with helper phage, which type of DNA can be produced, packaged and secreted into the medium?

Select the four statements about Plasmodium that are true. A)Inside an infected mosquito, haploid gametes fuse, forming a diploid zygote that develops into an oocyst B) Plasmodium cells infect two specific types of cells in humans. C) Inside an infected mosquito, haploid gametes fuse, forming a haploid zygote that develops into an oocyst. D) When a mosquito bites a human with malaria, diploid Plasmodium cells enter the mosquito. E) Plasmodium cells are transferred to humans through mosquito saliva. F) The life cycle of Plasmodium involves only haploid forms. G) The complete life cycle of Plasmodium requires two hosts.

Which of the following statements about immune response are correct?
P. T cells are activated by antigen-presenting cells.
Q. Foreign peptides are not presented to helper T cells by class II MHC proteins.
R. Dendritic cells are referred to as professional antigenpresenting cells.

Some strains of viruses can change normal mammalian cells into cancer cells in vitro. This transformation of the mammalian cell is usually associated with the

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

Read the following passage carefully and choose the most appropriate answer to the question out of the four alternatives.
The public sector banks are witnessing in india a period of transition and are at crossroads, where they without giving up social responsibility, should also remain healthy. They need to undertake risky experiments yet perform it innovatively in a way it does not fail. They should make forays into new areas which are rarely tread by them and lose no emerging opportunities. It should be understood that absence of any bad advance is no sign of efficient banking system. It only indicates immense conservatism. However this is no guarantee for profit. There should be a balance between liquidity and risk. Past sins should be forgotten. Novel and pragmatic techniques should be adopted without which banks would be in danger. What would happen if novel and pragmatic techniques are ignored?

When electroporation is used for introducing DNA into mammalian cell
P. A carrier for DNA is not required.
Q. The lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites.
R. The viability of the cell becomes approximately zero percent.
S. The first step involves absorption of DNA on the cell membrane.

Read the following passage carefully and choose the most appropriate answer to the question out of the four alternatives.
The public sector banks are witnessing in india a period of transition and are at crossroads, where they without giving up social responsibility, should also remain healthy. They need to undertake risky experiments yet perform it innovatively in a way it does not fail. They should make forays into new areas which are rarely tread by them and lose no emerging opportunities. It should be understood that absence of any bad advance is no sign of efficient banking system. It only indicates immense conservatism. However this is no guarantee for profit. There should be a balance between liquidity and risk. Past sins should be forgotten. Novel and pragmatic techniques should be adopted without which banks would be in danger. What according to the author, are the public sector banks witnessing.