The following are subunit vaccine produced by recombinant DNA technique

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

A

3, 1, 2, 4, 6, 5, 7, 8

B

1, 4, 3, 2, 5, 7, 6, 8

C

1, 2, 6, 7, 5, 8, 3, 4

D

8, 6, 7, 5, 1 , 4, 2, 3

Subunit, recombinant, polysaccharide and conjugate vaccine is which type of vaccine?

A

Biochemical vaccine

B

Biosynthetic vaccine

C

Natural vaccine

D

Biophysical vaccine

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

A

1, 2, 3 and 4 are true

B

Only 2 and 4 are true

C

Oniy 2, 3 and 4 are true

D

Only 1 and 2 are true

What happens when the gp130 subunit and the leukemia inhibitory factor receptor β-subunit is combined with a ciliary neurotrophic factor’s receptor subunit?

A

Changes the conformation of ciliary neurotrophic factor

B

Activates ciliary neurotrophic factor

C

Deactivates ciliary neurotrophic factor

D

A preference for ciliary neurotrophic factor developed

The only recombinant vaccine that proved highly effective and is currently approved for human use is the vaccine against __________

A

Rubella virus

B

Poxvirus

C

HPV infection

D

Dengue virus

Which of the following statements about DNA is/are correct?
1. DNA is the hereditary material of all living organisms.
2. All segments of DNA code for synthesis of proteins.
3. Nuclear DNA is double helical with two nucleotide chains which run anti-parallel.
4. DNA is also found in mitochondria.
Select the correct answer:

A

1, 2 and 3

B

3 and 4

C

1, 3 and 4

D

Only 4

The first recombinant vaccine produced targeted the surface antigen of which of the following virus?

A

DENV1

B

Ebola virus

C

Hepatitis A virus

D

Hepatitis B virus

Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because

A

The sequence context at the RB defines the direction of transfer

B

The sequence context at the LB defines the direction of transfer

C

The nuclear location sequence (NLS) of VirD2 protein drives the excised T-strand

D

The endonuclease activity of VirD2 protein allows nicking at RB

Which among the following is the classic isoform of the regulatory subunit of the catalytic PI3K subunit?

A

p87

B

p85

C

p89

D

p81

The eukaryotic subunit of RNA polymerase homologous to the ω subunit of bacterial RNA polymerase is ___________

A

RPA1

B

RPB2

C

RPC5

D

RPB6