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MRNA for microinjection into the Xenopus can be synthesized by in vitro ___________

Correct Answer: Transcription
Due to the large size of the oocytes, mRNA synthesized by transcription in vitro using phage T7-RNA polymerase can be used for microinjection.

You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

An in vitro translation system can synthesize peptides in all three reading frames of the RNA template. When 5' - UCUCUCUC-(UC)n-UCUCUCUC - 3' was used as the template in this in vitro translation system, the synthesized peptides contained 50% each of serine and leucine. When 5' - CCUCCUCCU-(CCU)n-CCUCCU - 3' was used as the template, the synthesized peptides contained 33.3% each of serine, leucine, and proline. Deduce the codon for proline.

The mRNA above was found to be much smaller than the original mRNA synthesized in the nucleus. This is due to the

A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro.

Proteins that are to be incorporated into cytoplasmic organelles are synthesized on ER membrane-bound ribosomes. Whereas, proteins that are to be imported into the ER-Golgi system are synthesized on free ribosomes in the cytosol.

Who synthesized the DNA in vitro?

RNA and DNA was synthesized in vitro by

In mammalian cells, RNA editing
P. Can change the coding capacity of a gene after transcription
Q. Creates a stop codon in the middle of the apo-B mRNA in intestinal cells but not in liver cells
R. Usually makes many changes in each mRNA
S. Can change specific nucleotides by deamination

Transgenic Drosophila can be created by microinjection of DNA into the embryos. These embryos are at which stage of development?