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Trypsin cleaves peptide bonds on the ‘c’ terminal side of the positively charged residues Arg and Lys, if the next residues is not _____

Correct Answer: Pro
Trypsin is a proteolytic enzyme with high specificity. It cleaves the peptide bond on the ‘c’ terminal side of the positively charged residues Arg and Lys, if the next residues is not Pro. It is used in the cleavage of protein molecules for protein sequencing.

The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be . . . . . . . . % and . . . . . . . . %.
Peptide I: Ala-Ala-Arg-Arg-Gln-Trp-Leu-Thr-Phe-Thr-Lys-Ile-Met-Ser-Glu
Peptide II: Ala-Ala-Arg-Glu-Gln-Tyr-Ile-Ser-Phe-Thr-Lys-Ile-Met-Arg-Asp

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:
• Amino acid composition of the peptide is: 2R, A, V, S, Y
• Trypsin digestion yields two fragments and the compositions are: (R, A, V) and (R, S, Y)
• Chymotrypsin digestion yields two fragments and the compositions are: (A, R, V, Y) and (R, S)
• Digestion with carboxypeptidase A yields no cleavage product.
Given: Trypsin cleaves at carboxyl side of R. Chymotrypsin cleaves at carboxyl side of Y. Carboxypeptidase A cleaves at amino side of the C-terminal amino acid (except R and K) of the peptide.
The correct amino acid sequence of the peptide is:

The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

Cyanogen bromide (CNBr) cleaves the peptide bond on the ‘C’ terminal side of the _______ residues.

You install Terminal Services on a Windows 2000 domain controller. You install Terminal Services Client on users' client computers. Users report that when they try to connect to the Terminal server, they receive the following error message: "The local policy of this system does not allow you to logon interactively." When you attempt to log on to the Terminal server as an administrator from a user's computer, you log on successfully. You want users to be able to log on to the Terminal server. What should you do?

Helix capping is the phenomenon in which, the side chains of which two flanking residues fold back to form hydrogen bonds with one of the four-terminal residues of the helix?

Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.

Six arms negotiators -- M,O,P,R,S and T-- are to be seated at a round table to discuss disarmament. There are exactly six chairs around the table. Each negotiator sits facing the center of the table and is directly opposite a negotiators across the table.The seating arrangement is subject to the following restrictions : T cannot sit next to P;S cannot sit next to M;R must sit next to M. If T sits next to M and S sits next to R, which of the other negotiators must sit next to s ?

Each of the questions below consists of a question and three statements numbered I, II and III given below it. You have to decide whether the data provided in the statements are sufficient to answer the question. Read all the three statements and give answer. There are six letters E, I, P, G, N and O is PIGEON the word formed after performing the following operations using these six letters only? I. O is placed fourth to the right of P. G is not placed immediately next to either P or O. II. N is placed immediately next (either left or right) to O. E is placed immediately next (either left or right) to G. III. Both I and E are placed immediately next to G. The word does not begin with N. P is not placed immediately next to E.

Six arms negotiators -- M,O,P,R,S and T-- are to be seated at a round table to discuss disarmament. There are exactly six chairs around the table. Each negotiator sits facing the center of the table and is directly opposite a negotiators across the table.The seating arrangement is subject to the following restrictions : T cannot sit next to P;S cannot sit next to M;R must sit next to M. If M sits next to P which of the following is a complete and accurate list of those who can sit next to P on P's other side ?